Journal: Animals : an Open Access Journal from MDPI

Article Title: Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent

doi: 10.3390/ani13132240

Figure Lengend Snippet: Sequence information of PCR primers.

Article Snippet: The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody (#2144, CST, Peachtree, GA, USA) was used as an internal reference.

Techniques: Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent

doi: 10.3390/ani13132240

Figure Lengend Snippet: Sequence analysis of Chicken RSPO1. ( A ) Alignment of amino acid sequences of chicken RSPO1 protein with other species. Shaded areas indicate homologous amino acids, black indicates 100% homology, pink indicates >75% homology, light blue indicates >50% homology. FU (furin-like): two cysteine-rich furin-like structural domains (marked with black box); TSP1: platelet response protein structural domain (marked with red box); LC (low complexity): low-complexity region. ( B ) Phylogenetic tree analysis of RSPO1 in chicken and other species of vertebrates.

Article Snippet: The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody (#2144, CST, Peachtree, GA, USA) was used as an internal reference.

Techniques: Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent

doi: 10.3390/ani13132240

Figure Lengend Snippet: Spatiotemporal expression of chicken RSPO1 . ( A ) Relative mRNA expression of RSPO1 in tissues of E12 (HH 38) male and female chicken embryos. n = 4. ( B ) Relative mRNA expression pattern of RSPO1 at different stages of chicken embryos, E12 =12 days of incubation. Beta-actin was used as a reference gene. n = 4. ( C ) Relative protein expression of RSPO1 in male and female gonads of E12 chicken embryos. Data are shown as mean ± SEM. Tubulin was used as a reference gene. n = 5. ( D ) RSPO1 mRNA expression levels in left and right gonads of E12 chick embryos. ( E ) Localization of RSPO1 protein expression in the ovaries and testes of E12 chick embryos. Scale bars = 50 μm. M: medulla. C: cortex.

Article Snippet: The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody (#2144, CST, Peachtree, GA, USA) was used as an internal reference.

Techniques: Expressing, Incubation

Journal: Animals : an Open Access Journal from MDPI

Article Title: Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent

doi: 10.3390/ani13132240

Figure Lengend Snippet: Effect of FAD treatment on the expression of RSPO1 in E12 chicken embryonic gonads. ( A ) Anatomy of male and female gonads of FAD-treated group and control group. L = left gonad, R = right gonad. Scale bars = 1cm. ( B ) Effect of FAD treatment on the mRNA expression of RSPO1 and some other genes in chicken embryonic gonads. Beta-actin was used as a reference gene. n = 4. ( C ) Effect of FAD treatment on the protein expression of RSPO1 in chicken embryonic gonads (the wrong symbol X indicates a false sample loading whose result was not included in the analysis). n = 4. Tubulin was used as a reference gene, and data are shown as mean ± SEM. FAD: fadrozole. FAD treatment was performed on E2.5 (embryonic day 2.5). E12 = embryonic day 12.

Article Snippet: The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody (#2144, CST, Peachtree, GA, USA) was used as an internal reference.

Techniques: Expressing, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent

doi: 10.3390/ani13132240

Figure Lengend Snippet: Effect of E2 treatment on the expression of RSPO1 in E10.5 chicken embryonic gonads. ( A ) Anatomy of male and female gonads of E2-treated group and control group. L = left gonad, R = right gonad. Scale bars = 1cm. ( B ) Effect of E2 treatment on the mRNA expression of RSPO1 and some other genes in chicken embryonic gonads. Beta-actin was used as a reference gene. n = 4. E2: estradiol. E2 treatment was performed on E2.5 (embryonic day 2.5). E10.5 = embryonic day 10.5.

Article Snippet: The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody (#2144, CST, Peachtree, GA, USA) was used as an internal reference.

Techniques: Expressing, Control

Journal: Animals : an Open Access Journal from MDPI

Article Title: Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent

doi: 10.3390/ani13132240

Figure Lengend Snippet: Expression of RSPO1 and estrogen pathway genes in gonadal cells and their response to E2. ( A ) Intrinsic expression of RSPO1 and estrogen pathway genes in gonadal cells. n = 6. ( B ) Effect of E2 treatment on RSPO1 mRNA expression in male gonadal cells. n = 5. male-E2-100/150/200/300 μM: male gonadal cells treated with 100/150/200/300 μM estradiol. Bars with different capital letters (A, B) are significantly different ( p < 0.05). ( C ) Effect of E2 treatment on mRNA expression of estrogen pathway genes in male gonadal cells ( n = 4). ( D ) Effect of E2 treatment on mRNA expression of RSPO1 in female gonadal cells ( n = 6). Beta-actin is used as a reference gene, and data are shown as mean ± SEM. E2: estradiol.

Article Snippet: The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody (#2144, CST, Peachtree, GA, USA) was used as an internal reference.

Techniques: Expressing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent

doi: 10.3390/ani13132240

Figure Lengend Snippet: Effects of RSPO1 overexpression on mRNA expression of downstream genes and estrogen pathway genes in gonadal cells. ( A ) RSPO1 mRNA expression in overexpression group and control group. n = 6. ( B ) Effect of RSPO1 overexpression on mRNA expression of WNT4 , CTNNB1 , and estrogen pathway genes in male gonadal cells. n = 6. Beta-actin was used as a reference gene, and data are shown as mean ± SEM.

Article Snippet: The RSPO1 antibody (AF3474) was purchased from R&D Systems (Minneapolis, MN, USA), and staining with a tubulin protein antibody (#2144, CST, Peachtree, GA, USA) was used as an internal reference.

Techniques: Over Expression, Expressing, Control

Journal:

Article Title: A novel and sensitive ELISA reveals that the soluble form of IL-13R-?2 is not expressed in plasma of healthy or asthmatic subjects

doi: 10.1111/j.1365-2222.2007.02921.x

Figure Lengend Snippet: sIL-13R-α2 detection in BAL fluid

Article Snippet: After static incubation for 2 h at 37 °C, plates were washed, and then incubated for 2 h, 37 °C, with biotinylated anti-human IL-13R-α2 (R&D Systems) diluted in PBS.

Techniques: